BioThema*專注于螢火蟲熒光素酶反應(yīng)的分析應(yīng)用，無論是檢測(cè)細(xì)胞，測(cè)量酶活性還是其他東西。在該反應(yīng)中，用ATP（三磷酸腺苷）活化熒光素，然后氧化成氧化熒光素和CO 2。氧化熒光素以激發(fā)態(tài)形成，并在進(jìn)入其基態(tài)時(shí)發(fā)光。通常在所謂的發(fā)光計(jì)中測(cè)量光。
ATP + 熒光素+ O 2 -熒光素酶 - > AMP +焦磷酸+氧化熒光素+ CO 2 +光
在ATP的測(cè)定中，光發(fā)射可以基本上恒定或快速衰減。因此，基于螢火蟲熒光素酶反應(yīng)，存在穩(wěn)定的光和閃光型ATP試劑。在熒光素酶水平低的情況下，每分鐘消耗的ATP比例可以忽略不計(jì)，然后光可以幾乎恒定但也很低。在高水平的熒光素酶下，ATP被消耗是不可避免的，導(dǎo)致光迅速減少，但初峰值光很高。實(shí)際上，峰高和衰減率都與熒光素酶活性成比例。BioThema開發(fā)了三種ATP試劑
BioThema2019年價(jià)格表
貨號(hào) | 品名 | 規(guī)格 | 價(jià)格 |
111-021 | Kinase RR Kit | 1000tests | 4879 |
144-041 | ATP Kit SL | 1000tests | 6035 |
155-050 | Cellular ATP Kit HTS | 1200tests | 7463 |
188-441 | Cell Viability Kit SL | 1600tests | 13073 |
266-111 | Intracellular ATP Kit HS | 375tests | 7769 |
266-112 | Microbial ATP Kit HS | 375tests | 7939 |
266-221 | ATP Hygiene Kit HS (dropper bottles) | 100tests | 3842 |
266-311 | ATP Biomass Kit HS | 375tests | 6341 |
484-001 | Luciferase Assay Kit | 800tests | 6902 |
484-102 | Luciferase Assay Kit HTS | 4800tests | 34578 |
484-103 | Luciferase Assay Kit HTS | 4600tests | 34578 |
PHL-192 | Portable Hygiene Laboratory | 192tests | 6970 |
11-501 | ATP Reagent SL | 250tests | 1156 |
11-501-M | ATP Reagent SL (excl. Mg2+) | 250tests | 1156 |
12-101 | ATP Reagent HS | 100tests | 646 |
12-071 | ATP Reagent HS | 17tests | 493 |
13-101 | ATP Reagent SS | 100tests | 3451 |
51-101 | ATP Substrate | 100tests | 442 |
51-201 | ATP Substrate | 200tests | 799 |
61-101 | Luciferin Substrate | 100tests | 442 |
61-201 | Luciferin Substrate | 200tests | 799 |
71-101 | ATP Eliminating Reagent | 500tests | 799 |
BT11-100 | D-Luciferin | 100mg | 867 |
BT11-500 | D-Luciferin | 500mg | 2754 |
BT11-1000 | D-Luciferin | 1000mg | 4420 |
BT12-100 | L-Luciferin | 100mg | 3026 |
BT11-100 K | D-Luciferin K salt | 100mg | 867 |
BT11-1000 K | D-Luciferin K salt | 1000mg | 4420 |
BT11-100 Na | D-Luciferin Na salt | 100mg | 867 |
BT11-1000 Na | D-Luciferin Na salt | 1000mg | 4420 |
LA-10 | Firefly Luciferase (recomb., enhanced activity) | 10mg | 13787 |
47-051 | ATP Standard | 5mL | 340 |
46-051 | ATP Standard | 5mL | 340 |
45-051 | ATP Standard | 5mL | 340 |
48-072 | ATP Standard (dropper bottle) | 6.7mL | 527 |
21-101 | Tris-EDTA Buffer | 10ml | 153 |
21-103 | Tris-EDTA Buffer | 100ml | 782 |
21-500 | Tris-EDTA Buffer | 500ml | 2550 |
22-101 | Diluent B | 10ml | 340 |
22-072 | Diluent B (dropper bottle) | 6.7ml | 289 |
23-101 | Diluent C (SL) | 10ml | 340 |
24-101 | Diluent D | 10ml | 340 |
27-101 | ATP Free H2O | 10ml | 323 |
40-500 | ATP Diluent | 10ml | 425 |
31-072 | Extractant B/S (dropper bottle) | 6.7ml | 408 |
31-101 | Extractant B/S | 10ml | 595 |
31-103 | Extractant B/S | 100ml | 3043 |
31-500 | Extractant B/S | 500ml | 9163 |
20-121 | Lysing Diluent | 12ml | 408 |
28-101 | Cell Lysing Reagent | 10ml | 884 |
681000 | Cuvettes with 'stirring wings' | 1000pcs | 2380 |
61303 | LuciPac W | 100 tests | 4573 |
60331 | LuciPac Pen | 100 tests | 4573 |
| CheckLite 250 Plus | 250 tests | 6511 |
| CheckLite HS Set | 100 tests | 6511 |
| CheckLite CT150 | 150 tests | 7531 |
LA-11 | Firefly Luciferase (recomb., thermostable) | 10 mg | 6086 |
LA-12 | Firefly Luciferase (recomb., thermostable) | 100 mg | 24752 |
PD-20 | Lumitester™ handluminometer PD-20 | ea | 17697 |
PD-30 | Lumitester™ handluminometer PD-30 | ea | 18751 |
C-110 | Lumitester™ portable luminometer C-110 inc | ea | 86615 |
DPU-H245 | Printer set for LUMITESTER C-110 Includes following items | ea | 5661 |
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